Institute for Medical Research
Centre of Research Excellence in Nutrition and Metabolism
Tadeuša Košćuška 1, 11 000 Beograd
Phone number: 011 406 17 47
e-mail: irenakrga@yahoo.com

Education

• 2014-2018. Doctoral studies (Integrated Nutrition Sciences), Faculty of Biology, University of Belgrade, Belgrade, Serbia
• 2014.-2018. Doctoral studies (Human Nutrition), Faculty of Medicine, University Clermont Auvergne, Clermont Ferrand, France (international programme of joint supervision of doctoral thesis (“Cotutelle”))
  Thesis title: “The effect of anthocyanins and their metabolites on the function of human endothelial cells and platelets in vitro”
• 2013. MCs in Ecology, Faculty of Biology, University of Belgrade, GPA 10.00/10.00
• 2012 BCs in Biology, Faculty of Biology, University of Belgrade, GPA 9.63/10.00
• 2004-2008. 9th Gymnasium, Belgrade

Work experience

• 02.04.2018.-today. Institute for Medical Research, University of Belgrade, Centre of Research Excellence in Nutrition and Metabolism, Belgrade, Serbia
• November 2014.-November 2017. PhD fellow, National Institute of Agricultural Research (INRA),Human Nutrition Unit, Micronutrients and Cardiovascular health team, Theix, France

Projects

National projects:

• Biological effects, nutritional intake and status of folate and polyunsaturated fatty acids: The improvement of nutrition in Serbia”, National project (no. III41030, 2011-)

International projects:

• European Cooperation in Science and Technology (COST) action FA1403 POSITIVe- “Interindividual variation in response to consumption of plant food bioactives and determinants involved” (2014-2018)
  Courses
• IUBMB Advanced School on Nutrition, Metabolism and Aging. 15th- 19th October 2018, Petnica Science Center, Petnica, Serbia
• “Assessments of Ser/Thr Kinase activity by PamGene Station”, Short-term scientific mission 13.03.2017- 10.04.2017, Proteinchemistry, Proteomics & Epigenetic Signaling (PPES), University Antwerp, Antwerp, Belgium
• “Use of nutrigenetics & nutri(epi)genomics in nutrition“, 2nd Training school of COST Action POSITIVe, 20.-22.09.2017., Thessaloniki, Greece
• Communication skills, 04.-05.01.2016, Doctoral School for Life Sciences, Health, Agronomy and Environment, Clermont-Ferrand, France
• Project management, 21.-22.01.2016, Doctoral School for Life Sciences, Health, Agronomy and Environment, Clermont-Ferrand, France

Research skills and techniques

• Cell culturing (primary endothelial cells, monocytic cell lines), cell-based assays: cell adhesion, cell migration, viability assays
• DNA and RNA extraction, agarose-gel electrophoresis, RT-qPCR, TaqMan Low Density Arrays, miRNA microarrays
• Protein extraction, protein purification, quantification, Western Blotting, Ser/Thr Kinase activity assays
  Immunostaining, flow cytometry
• Fluorescent microscopy
• Measurements of biochemical and hematologic parameters
• Bioinformatics analyses (transcription factor and pathway analyses)

Software skills

• GeneSpring (Agilent), Relative Quantitation software (ThermoFisher), MetaCore (GeneGo)
• Prism (GraphPad), SPSS (IBM)
• Cell Quest Pro (flow cytometry)
• ImageJ (NIM)
• Microsoft Office – Word, Excel, PowerPoint, Adobe Photoshop CS

Language skills

• English, advanced level
• French, Intermediate level

Honors

• French government grant for co-tutoring doctorate (2014-Present)
• Scholarship “Dositeja”, Young talents Fund

Publications

International journals
• I. Krga, D. Milenkovic (2019). Anthocyanins: from sources and bioavailability to cardiovascular health benefits and molecular mechanisms of action. J Agric Food Chem., 67(7):1771-1783.(M21a, IF2018 3.571)
• I. Krga, R. Tamaian, S. Mercier, C. Boby , LE Monfoulet, M Glibetic , C Morand , D. Milenkovic (2018). Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability, Free Radical Biology and Medicine, 124, 364-379. (M21, IF2017 6.020)
• I. Krga, N. Vidovic, D. Milenkovic, A. Konic-Ristic, F. Stojanovic, C. Morand, M. Glibetic, Effects of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and their aggregation with monocytes and neutrophils, Archives of Biochemistry and Biophysics, 645 (2018) 34-41. (M22, IF2016 3.165)
• I. Krga, D. Milenkovic, C. Morand, L.E. Monfoulet, An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols, Food & Function, 7 (2016) 3656–3676. (M21, IF2016 3.247)
• I. Krga, L.E. Monfoulet, A. Konic-Ristic, S. Mercier, M. Glibetic, C. Morand, D. Milenkovic, Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNFα-activated endothelial cells at physiologically relevant concentrations, Archives of Biochemistry and Biophysics, 599 (2016) 51-59. (M22, IF2016 3.165)
National journals
• I. Krga, D. Pavlović, A. Anđelković, S. Đurović, D. Marisavljević. Osetljivost korovskih populacija u voćnjacima na glifosat, Zaštita bilja, 64 (2013) 125-133.

Oral presentations
• I. Krga. 5th Serbian Biomarker Symposium (SERBIS 2019), Polyphenols and cardiovascular health 26th -28th March 2019, Belgrade, Serbia
• I. Krga, R. Tamaian, S. Mercier, C. Boby, C. Morand, A. Pantovic, M. Glibetic, D. Milenkovic. Dietary anthocyanins and their metabolites decrease monocyte adhesion and migration across activated endothelial cells through mechanisms that regulate endothelial permeability. IUBMB Advanced School on Nutrition, Metabolism and Aging. 15th- 19th October 2018, Petnica Science Center, Petnica, Serbia, p. 16. (Best oral presentation award)
• I. Krga, R. Tamaian, S. Mercier, C. Boby, C. Morand, M. Glibetic, D. Milenkovic. Dietary anthocyanins and their metabolites lower monocyte adhesion and diapedesis through mechanisms that regulate endothelial cell permeability. UNIFood Conference, Univeristy of Belgrade 210th Aniversary. 5th-6th October 2018, Belgrade, Serbia, FH3
• I. Krga, S. Mercier, R. Tamaian, C. Boby, L-E. Monfoulet, C. Morand, D. Milenkovic. Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability. 8th International Conference on Polyphenols and Health, 3rd-6th October 2017, Quebec City, Canada.
• I. Krga. Effect of anthocyanins and their metabolites on endothelial and platelet function. Proteinchemistry, 17.03.2017, Proteomics & Epigenetic Signaling (PPES), University Antwerp, Antwerp, Belgium
• I. Krga. Effect of physiologically relevant concentrations of anthocyanins and their gut metabolites on adhesion of monocyte to TNFα-activated endothelial cells. Doctoral school days, 2nd– 3rd June 2016, Clermont-Ferrand, France
• I. Krga, S. Mercier, L-E. Monfoulet, C. Morand, A. Konic-Ristic, D. Milenkovic. Effect of physiologically-relevant concentrations of anthocyanins and their gut metabolites on monocyte adhesion to endothelial cells. 2nd Interregional annual conference on Nutrition and metabolism, Rhône-Alpes-Auvergne, 4th-5th November 2015, Saint Galmier, France (p.35).

Poster presentations
• I. Krga, N. Kardum, A. Konić Ristić, D. Milenkovic, A. Pantović, M. Glibetic. Impact of dietary phenolic acids on agonist-induced markers of platelet activation. UNIFood Conference, Univeristy of Belgrade 210th Aniversary. 5th-6th October 2018, Belgrade, Serbia, FHP10.
• I. Krga, N. Kardum, F. Stojanovic, A. Konic-Ristic, D. Milenkovic M. Glibetic. Effects of physiologically-relevant concentrations of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and aggregation. 8th International Conference on Polyphenols and Health, 3rd-6th October 2017, Quebec City, Canada (P04-071).
• I. Krga, R. Tamaian, C. Boby, S. Mercier, C. Morand, D. Milenkovic. Anthocyanins and their gut metabolites reduce monocyte adhesion and migration across TNF-activated endothelial cells through nutrigenomic mechanisms regulating endothelial integrity. 3rd Scientific Workshop: “Omics breakthroughs in the health effects of plant food bioactive”, 20th-21stSeptember 2017, Thessaloniki, Greece (P8, Best poster award).
• Konic-Ristic, I. Krga, N. Kardum, D. Milenkovic, M. Glibetic. Effects of physiologically-relevant concentrations of anthocyanins and their metabolites on adenosine diphosphate-induced platelet activation and aggregation. 1st Congress of Molecular Biologists of Serbia (CoMBoS). 20th-22nd September 2017, Belgrade, Serbia (p.144).
• I. Krga, C. Morand, C. Boby, L.E. Montfoulet, M. Glibetic, D. Milenkovic. Mixtures of anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability. 1st Congress of Molecular Biologists of Serbia (CoMBoS). 20th-22nd September 2017. Belgrade, Serbia (p. 50).
• I. Krga, S. Mercier, L-E. Monfoulet, C. Morand, A. Konic-Ristic, D. Milenkovic. Effect of anthocyanins and their microbiota metabolites on monocyte adhesion to endothelial cells at physiologically relevant concentrations, CRNH Auvergne Scientific day, 26th November 2015, Clermont-Ferrand, France (P02).
• I. Krga, L.E. Monfoulet, A. Konic-Ristic, S. Mercier, M. Glibetic, C. Morand, D. Milenkovic. Effect of anthocyanins and their gut metabolites on monocyte adhesion to endothelial cells at physiologically-relevant concentrations. 7th International Conference on Polyphenols and Health, 27th-30th October 2015, Tours, France (P615, p.220).