Laboratory for dietary intervention studies with experimental kitchen

The main activities of the Laboratory for Dietary Intervention Studies of CENM include the design and conduction of dietary intervention studies aiming to elucidate the effects of nutrients and non-nutritive dietary compounds in the prevention of chronic diseases, with the special focus on the prevention of cardiovascular diseases (CVD).
The core of the research activities are randomised, double blind, placebo controlled dietary intervention studies, with parallel and cross-over assignment as intervention models.
Based on the purpose the studies involve:

  1. Healthy volunteers – in studies targeted to the primary prevention.
  2. Subjects at high risk for CVD including obese, hyperlidemic, hyperglycemic subjects, subjects with metabolic syndrome, high blood pressure or healthy subjects with unhealthy life style (ie. smoking, unhealthy dieatry regiments) – in studies targeted to the secondary prevention.
  3. Subjects with CVD or diabetes – in studies targeted to the tertiary CVD prevention (prevention of complications of the diseases).

Based on the endpoint classification two main types of studies include safety and efficacy studies.

Biomarkers and risk factors assessed within the proof-of concept dietary intervention studies include:

  1. Anthropometric parameters (weight, BMI, waist circumference, body fat, body water, lean mass)
  2. Biochemical parameters (status of lipid and glucose metabolism, folate status, antioxidant/prooxidant status, inflammatory status, serological markers of endothelial function)
  3. Blood pressure and other markers of endothelial function
  4. Biomarkers of cellular status and function:
  • Markers of platelet activation and their aggregation with monocytes and neutrophils by using flow cytometry
  • Lipid status of cellular membranes (in erytrocytes, platelets, lymphocytes) by using gas chromatography
  • Antioxidant and anti-inflammatory status (in erytrocytes, monocytes, platelets, lymphocytes) by using flow cytometry, ELISA and spectroscopic methods

Laboratory for Dietary Intervention Studies is supported with the research activities of other laboratories within CENM in the assessment of dietary intake of nutrients and food bioactives (FFQ, 24h-recalls, food diaries) and physical activity assessment, statistical analysis of the obtained data and dissemination of the results.

Projects:

EC Projects

  • FP7: “Bacchus- Beneficial effects of dietary bioactive peptides and polyphenols on cardiovascular health in humans” (2011 – 2015), coordinated by Institute of Food Research, Norwich
  •  FP7: “BaSeFood- Sustainable exploitation of bioactive components from the Black Sea Area traditional foods” (2009 – 2012), coordinated by University of Bologna

Bilateral projects

  • Bilateral Collaboration Serbia-France “Pavle Savić”: “Identification of cellular and molecular targets of plant bioactives on human blood cells: clinical and in-vitro studies” (IMR-INRA); (2012-2013)

National projects

  • Biological mechanisms, dietary intake and status of polyunsaturated fatty acids and folates: Improvement of nutrition in Serbia; funded by the Ministry of Educ, Sci & Technol Devel of the Republic of Serbia (MNTRS), No. 46030, 2011-2014 (PI: Dr. Maria Glibetić).

References:

  • Konić Ristić A., Srdić-Rajić T., Kardum N. and Glibetić M. (2013). Biological activity of Aronia melanocarpa antioxidants pre-screening in an intervention study design J Serb Chem Soc, 78(3), 429–443.
  • Konić-Ristić A., Srdić-Rajić T., Kardum N., Aleksić-Veličković V., Kroon P.A., Hollands W.J., Needs P.W., Boyko N., Hayran O., Jorjadze M. and Glibetić M. (2013). Effects of bioactive-rich extracts of pomegranate, persimmon, nettle, dill, kale and Sideritis and isolated bioactives on arachidonic acid induced markers of platelet activation and aggregation. J Sci Food Agric, doi:10.1002/jsfa.6328. (IF: 1.759)
  • Glibetic M, Konic-Ristic A, Srdic-Rajic T, Kardum N, Kroon PA, Hollands W, Boyko N. Acute effects of hot water infusions of mountain tea, nettle and dill on platelet function in subjects with metabolic syndrome: a randomised controlled intervention study. Traditional Food International 2012, 4-5th October, 2012., Cesena, Italy
  • Kardum N, Konic-Ristic A, Glibetic M, Srdic-Rajic T, Kroon PA, Hollands W, Woodcock M, Boyko N. Effects of 6 weeks of pomegranate juice consumption on cardiovascular disease risk biomarkers in subjects with metabolic syndrome: a randomised controlled intervention study. Traditional Food International 2012, 4-5th October, 2012., Cesena, Italy.
  • Konic-Ristic A, Stanojkovic T, Srdic-Rajic T, Zdunic G, Savikin K, Glibetic M. Anti-proliferative and cell-cycle effects of chokeberry and pomegranate juices on colon cancer cells in vitro. 11th FENS European Nutrition Conference, 26-29th October, 2011, Madrid, Spain. Abstract book, p.364, pp. 27/783.
  • Srdic-Rajic T, Konic-Ristic A, Arsić A, Zec M, Stojanović F, Kardum D. Effect of acute intake of Aronia melanocarpa juice on antioxidative status and platelet function-ex vivo study. 11th FENS European Nutrition Conference, 26-29th October, 2011, Madrid, Spain. Abstract book, p.339, pp.27/473.
  • Konic-Ristic A, Srdic-Rajic T, Arsić A, Djekić Ivanković M, Kardum D, Glibetić M. Effect of long-term consumption of Aronia melanocarpa juice on antioxidative status of patients with metabolic disease. 5th International Conference on Polyphenols and Health, 17-20th October, 2011, Sitges, Spain. Ab. book, pp 509.

PhD Theses:

  • The impact of plant polyphenols on platelet activation and their aggregation with blood, malignant and endothelial cells- Aleksandra Konić Ristić, PhD Thesis, Faculty of Pharmacy, University of Belgrade, Serbia, 2013
  • Nevena Kardum: The impact of chokeberry juice consumption on antioxidant/prooxidant status and fatty acid profiles in healthy subjects and subjects at high cardiovascular diseases risk – Faculty of Pharmacy, University of Belgrade, Serbia, due to 2014


Dr Maria Glibetic

SOULFOOD Serbia

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